CryoET Specialist

The Hormel Institute at the University of Minnesota is seeking a Research Specialist to join the CryoEM facility. This role supports the local research community at The Hormel Institute, as well as external academic and industry partners. The position works under general supervision and exercises discretion to make daily operational decisions based on best practices.

Focus of Job Responsibilities

Research Design and Operation of Associated Instruments & Protocols - 50%

* Manage and optimize use of the Arctis
* Develop workflows that links sample preparation (Arctis) to data collection (Krios).
* Develop and optimize sample and data collection parameters.
* Develop protocols for SEM and IFLM sample screening, milling of vitrified samples, waffle milling and cryo sectioned samples.
* Optimize protocols for slice and view function.
* Operate Titan Krios as needed and provide support for tomography data collection.
* Prepare grids and reagents for ET experiments including carbon coated grids for negative stain, graphene/continuous carbon grids for cryoEM, negative stains and fiducials.
* Perform conventional vitrification using the Vitrobot, CP2, Leica GP2 and slammer.
* Culture live cells on grids for cryoET experiments, apply adhesive coatings to grids for cell culture, and prepare grids for cell culture via micropatterning. Additionally, develop protocols for frequently used cell lines and making a library of preferred conditions.
* Perform live cell vitrification using both conventional vitrification instruments, and high pressure freezing for standard FIB-milling and the Waffle technique.
* Perform vitrification of tissue samples using a high-pressure freezer prepared through various means such as microtome.
* Troubleshoot experimental approaches for a variety of different samples and experiments to meet users needs.
* Instrumental operation including image acquisition and microscope maintenance.
* Implement data collection strategies that will benefit our user community.

Problem Resolution - 25%

* Exercise considerable resourcefulness to find solutions to research issues within budgetary constraints.
* Applies subject matter knowledge, including research technologies, to ensure operational effectiveness.
* Lead workshop development and functions.
* Assign duties to technical support staff.
* Demonstrate decision-making that is guided by policies and procedures created by the cryo-EM team.
* Serve as a liaison with internal and external contacts regarding research.
* Modify policies and procedures to improve efficiency and quality.
* Proactively search for a better way to effectively achieve end results.
* Achieve a scope of measurable impact.

3D Data Processing, Reconstruction, & User Support - 25%

* Collect and process data for 3D map and tomogram reconstruction.
* Produce subtomogram averages and segmentations as needed.
* Provide experimental and analytical support to users.
* Engage in data analysis including image analysis, data processing and reconstruction.
* Perform single particle analysis reconstruction using CryoSPARC and Relion.
* Perform tomogram reconstruction, segmentation and subtomogram averaging using WarpTools, Imod, Aretomo and Chimera/ChimeraX.
* Engage with users to train and provide experimental support in sample preparation such as negative stain, culturing cells on grids and vitrification.
* Operate instruments such as Vitrobot, High Pressure Freezer and Arctis, Krios.
* Analyze data through tomogram reconstruction, segmentation, subtomogram averaging and single particle analysis.
* Provide support in experimental set-up or considerations for single particle and tomography experiments.
* Serve as the lead on assigned projects.

Flexible Work Location Profile
The Research and Innovation Office (RIO) and The Hormel Institute endorses a "work with flexibility" approach that offers a welcoming and flexible work environment where everyone is inspired to do their best. Work location options include working fully remote, partially remote, or entirely in the office and are based on the work of the position. Some on-site work may be necessary for certain positions, even those designated as fully remote. Because we are a land-grant institution that serves the state, the University will continue to- in most cases- expect employees to live in Minnesota.

This position has been designated as eligible for Flexible Work Profile One.
Flexible Work Profile One is expected to work primarily on-site. Your office will be located at The Hormel Institute in Austin, MN. The Hormel Institute management retains the right to modify flexible work arrangement agreements on a temporary or permanent basis for any reason at any time.

Required Qualifications

* Ph.D., DDS, DVM, JD, MD or equivalent
* Proven experience with fundamental biochemistry and molecular biology techniques.
* Experience in structural biology, with emphasis on cryo-electron tomography.

Preferred Qualifications

* Ph.D. in chemistry or biochemistry preferred.

The Hormel Institute is an independent biomedical research institution within the University of Minnesota's Research and Innovation Office. Established in 1942, our mission is to conduct research and provide education in the biological sciences with applications in medicine and agriculture, specifically by understanding, treating, and controlling cancer and other chronic diseases. The Hormel Institute serves as a center of technical and educational expertise for the benefit of the local community, the State of Minnesota, and beyond. We are located in the uniquely diverse rural community of Austin, MN, conveniently only 35 miles from Rochester, MN, and 100 miles (1.5 hours) south of Minneapolis/St. Paul. The Hormel Institute is committed to fostering a work environment that promotes inclusive excellence, supportive employee practices, and encourages diversity of thought that drives innovation and creativity in our science.

Pay Range: $81,037- $92,232.28; depending on education/qualifications/experience

Time Appointment Category: 75%-100% Appointment (If job description above does not specify appointment or expected weekly hours, assume a 100% appointment.)

Position Type: Faculty and P&A Staff

Please visit the Office of Human Resources website for more information regarding benefit eligibility.

The University offers a comprehensive benefits package that includes:

• Competitive wages, paid holidays, and generous time off
• Continuous learning opportunities through professional training and degree-seeking programs supported by the Regents Tuition Benefit Program
• Low-cost medical, dental, and pharmacy plans
• Healthcare and dependent care flexible spending accounts
• University HSA contributions
• Disability and employer-paid life insurance
• Employee wellbeing program
• Excellent retirement plans with employer contribution
• Public Service Loan Forgiveness (PSLF) opportunity
• Financial counseling services
• Employee Assistance Program with eight sessions of counseling at no cost
• Employee Transit Pass with free or reduced rates in the Twin Cities metro area

Applications must be submitted online. To be considered for this position, please click the Apply button and follow the instructions. You will have the opportunity to complete an online application for the position and attach a cover letter and resume.

Additional documents may be attached after application by accessing your "My Job Applications" page and uploading documents in the "My Cover Letters and Attachments" section.

This position will remain open until filled.

To request an accommodation during the application process, please e-mail employ@umn.edu or call (612) 624-8647.

The University recognizes and values the importance of diversity and inclusion in enriching the employment experience of its employees and in supporting the academic mission. The University is committed to attracting and retaining employees with varying identities and backgrounds.

The University of Minnesota provides equal access to and opportunity in its programs, facilities, and employment without regard to race, color, creed, religion, national origin, gender, age, marital status, disability, public assistance status, veteran status, sexual orientation, gender identity, or gender expression. To learn more about diversity at the U: http://diversity.umn.edu

Any offer of employment is contingent upon the successful completion of a background check. Our presumption is that prospective employees are eligible to work here. Criminal convictions do not automatically disqualify finalists from employment.

The University of Minnesota, Twin Cities (UMTC)

The University of Minnesota, Twin Cities (UMTC), is among the largest public research universities in the country, offering undergraduate, graduate, and professional students a multitude of opportunities for study and research. Located at the heart of one of the nation's most vibrant, diverse metropolitan communities, students on the campuses in Minneapolis and St. Paul benefit from extensive partnerships with world-renowned health centers, international corporations, government agencies, and arts, nonprofit, and public service organizations.

At the University of Minnesota, we are proud to be recognized by the Star Tribune as a Top Workplace for 2021, as well as by Forbes as Best Employers for Women and one of America's Best Employers (2015, 2018, 2019, 2023), Best Employer for Diversity (2019, 2020), Best Employer for New Grads (2018, 2019), and Best Employer by State (2019, 2022).